Characterization of living normal and leukemic mouse lymphocytes by fluorescein diacetate.

Osmotic behavior of normal and leukemic lymphocytes.

The response of normal human peripheral blood lymphocytes to a hypotonic environment may be divided into two phases: the cells first exhibit rapid osmotic swelling, followed by a slower shrinking phase, during which they regain their initial physiologic volume. This osmotic behavior is characteristic of most mammalian and avian nucleated cells so far examined. The normal human blood lymphocyte,...

Protein Concentration in Normal Mouse Lymphocytes, Thymocytes, and Mouse and Human Leukemic Cells as Measured by Interference Microscopy

The apparent protein content of a single spherical lymphocyte or a similar cell, as well as its diameter, can be measured by a modified and stabilized AO-Baker interference microscope which is either fitted with an AO half-shade eyepiece or connected to a photomultiplier. This paper gives results for the apparent protein content and the diameter of normal mouse lymphocytes and thymocytes; these...

The surface ultrastructure of normal and leukemic rat lymphocytes.

In studies on the nature of the malignant state, there has been increasing interest in the external surface of cancer cells. Coman and his co-workers (1, 2, 5) established the fact that the intercellular adhesiveness of malignant cells was lower than that of their normal prototypes and that this difference probably played a major role in the invasive characteristics of cancer cells. Since ad he...

Short Communication Characterization of Recombinant Human Carboxylesterases: Fluorescein Diacetate as a Probe Substrate for Human Carboxylesterase

Human carboxylesterase (CES) 1 and CES2 are members of the serine hydrolase superfamily, and both exhibit broad substrate specificity and are involved in xenobiotic and endobiotic metabolism. Although expression of CES1 and CES2 occurs in several organs, their expression in liver and small intestine is predominantly attributed to CES1 and CES2, respectively. We successfully expressed CES1 form ...

Characterization of Recombinant Human Carboxylesterases - Fluorescein Diacetate as a Probe Substrate for Human Carboxylesterase